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  • Gbona ta Flag cas 98849-88-8

Jul. 28, ọdun 2023 10:27 Pada si akojọ

Gbona ta Flag cas 98849-88-8



111 Gbona ta Flag cas 98849-88-8

222 FLAG peptide jẹ ẹya mẹjọ-amino acid polypeptide (asp-ty-rlys-asp-asp-asp-asp-asp-Lys) pẹlu aaye gige-enterokinase. FLAG peptide ni a lo fun idanimọ ati isọdọmọ ti awọn ọlọjẹ atunmọ ti o ni ilaja nipasẹ awọn ọlọjẹ

333 Imọ-ẹrọ amuaradagba Fusion ti di ohun elo pataki lati yanju ọpọlọpọ awọn iṣoro ti o ni ibatan si iṣelọpọ amuaradagba atunlo. Iseda ti aami afikun ṣe iranlọwọ lati ṣe idanimọ ati pese ilana isọdọmọ-igbesẹ kan fun amuaradagba idapọ nipa gbigbe jade sẹẹli tabi alabojuto nipasẹ ọwọn ti awọn sobusitireti ti o yẹ. A gba peptide FLAG laaye lati yọ jade labẹ awọn ipo ti kii ṣe denaturing. Orisirisi awọn egboogi lodi si peptide FLAG ti ni idagbasoke. Antibody M1 sopọ mọ peptide kan niwaju cation irin divalent, ni pataki Ca2 +. Elution nipa chelating oluranlowo. Ilana miiran jẹ elution ifigagbaga pẹlu apọju FLAGE peptides ọfẹ. Awọn egboogi M2 ati M5 ni a lo ninu ilana yii [1]. Aami Flag ni akọkọ ṣe apejuwe bi apẹrẹ ti o gbẹkẹle kalisiomu ti egboogi monoclonal kan. O jẹ octapeptide ekikan ti o ga julọ ti o le dapọ pẹlu amuaradagba afojusun ni N-terminal. Gẹgẹbi peptide hydrophilic pupọ, aami Flag ni iṣeeṣe dada giga kan. Amuaradagba Flag-fusion le jẹ igbasilẹ nipasẹ awọn ọwọn imunoaffinity ni iwaju Ca2 + ati yọkuro nipasẹ EDTA ni awọn ifọkansi kekere, pH didoju ati nitorinaa labẹ awọn ipo iṣe-ara

Awọn ohun-ini kemikali ti FLAG peptide

Ilana molikula C41H60N10O20

Molikula àdánù 1012.97000

Iwọn deede 1012.40000

PSA 525.79000

Flag Peptide TFA iyọ. Flag peptide TFA Iyọ

Nọmba CAS 98849-88-8 Iwọn Molikula 1012.97000

Ìwúwo N/A Ojuami farabale N/A

Ilana molikula C41H60N10O20 Ojuami Iyọ N/A

MSDS N/A Flash ojuami

FLAG inagijẹ ti peptide

1.1 Flag Peptide
1.2 Flag EPITOPE PEPTIDE
1.3
1.4 Flag tag Peptide
1.5 ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
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Asset 3

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