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Jul. 14, 2023 10:14 Hoʻi i ka papa inoa

Kūʻai wela ʻo FLAG cas 98849-88-8



111 Kūʻai wela i ka HAE cas 98849-88-8

ʻO 222 FLAG peptide he ʻewalu amino acid polypeptide (asp-ty-rlys-asp-asp-asp-asp-asp-Lys) me kahi kahua ʻoki enterokinase. Hoʻohana ʻia ʻo FLAG peptide no ka ʻike a me ka hoʻomaʻemaʻe ʻana i nā protein recombinant i hoʻopili ʻia e nā antibodies.

Ua lilo ʻo 333 Fusion protein i mea hana koʻikoʻi no ka hoʻoponopono ʻana i nā pilikia he nui e pili ana i ka hana ʻana i ka protein recombinant. ʻO ke ʻano o ka lepili hou e kōkua i ka ʻike a hāʻawi ʻana i kahi kaʻina hana hoʻomaʻemaʻe hoʻokahi no ka protein fusion ma o ka hoʻohele ʻana i ka cell extract a i ʻole supernatant ma o ke kolamu o nā substrate kūpono. ʻAe ʻia ka peptide FLAG e ʻeluate ma lalo o nā kūlana non-denaturing. Ua kūkulu ʻia kekahi mau antibodies e kūʻē i ka peptide FLAG. Hoʻopili ʻia kahi antibody M1 i kahi peptide i mua o kahi cation metala divalent, ʻoi aku ka maikaʻi o Ca2 +. Elution by chelating agent. ʻO kahi hoʻolālā ʻē aʻe he elution hoʻokūkū me nā peptides FLAGe manuahi ʻoi aku. Hoʻohana ʻia nā antibody M2 a me M5 i kēia kaʻina hana [1]. Ua wehewehe mua ʻia ka lepili Hae ma ke ʻano he epitope hilinaʻi calcium o kahi antibody monoclonal. He octapeptide ʻakika loa ia e hiki ke hoʻokuʻi me ka protein target ma ka N-terminal. Ma ke ʻano he peptide hydrophilic, ʻoi aku ka nui o ka lepili Flag. Hiki ke hopu ʻia ka protein Flag-fusion e nā kolamu immunoaffinity i mua o Ca2 + a hoʻoheheʻe ʻia e EDTA ma nā haʻahaʻa haʻahaʻa, pH kūʻokoʻa a no laila ma lalo o nā kūlana physiological.

Nā waiwai physicochemical o FLAG peptide

ʻAno molekole C41H60N10O20

Kaumaha Molekala 1012.97000

Nuipa pololei 1012.40000

PSA 525.79000

FLAG Peptide TFA paʻakai. FLAG peptide TFA PAʻakai

Helu CAS 98849-88-8 Kaumaha molekula 1012.97000

Kaha N/A Puni paila N/A

Māmā molekala C41H60N10O20 Kahue hehee N/A

MSDS N/A wahi Flash

FLAG Alias ​​o ka peptide

1.1 HAE Peptide
1.2 HAE EPITOPE PEPTIDE
1.3 Trypsinogen (kanaka pancreas isoenzyme 2 activation peptide), 1-L-aspartic acid-2-L-tyrosine-3-L-lysine-
1.4 FLAG tag Peptide
1.5 ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
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