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  • Ukuthengisa okushushu kweFLAG cas 98849-88-8

Jul . 14, 2023 10:14 Buyela kuluhlu

Ukuthengisa okushushu kweFLAG cas 98849-88-8



111 Ukuthengisa okushushu kweFLAG cas 98849-88-8

I-222 FLAG peptide yi-polypeptide ye-amino acid esibhozo (i-asp-ty-rlys-asp-asp-asp-asp-Lys) enendawo yokusika i-enterokinase. I-FLAG peptide isetyenziselwa ukuqatshelwa kunye nokuhlanjululwa kweeprotheni eziphinda ziphinde zihlangane nezithinteli-zifo.

I-333 iteknoloji yeprotheyini ye-Fusion iye yaba sisixhobo esibalulekileyo sokusombulula iingxaki ezininzi ezinxulumene nokuveliswa kweprotheyini edibeneyo. Ubume beleyibhile eyongezelelweyo inceda ukuchonga kunye nokubonelela ngenkqubo yokucoca inyathelo elinye kwiprotheni yokudibanisa ngokudlula i-cell extract okanye i-supernatant ngokusebenzisa ikholamu yee-substrates ezifanelekileyo. I-peptide ye-FLAG ivumelekile ukuba ivuleke phantsi kweemeko ezingeyo-denaturing. Ziliqela izilwa-buhlungu ezichasene ne-FLAG peptide zenziwe. I-antibody M1 ibophelela kwi-peptide xa kukho i-divalent metal cation, ngokukhethekileyo i-Ca2 +. I-elution nge-arhente ye-chelating. Esinye isicwangciso kukukhuphisana elution kunye neepeptides zasimahla zeFLAGE. Ii-antibodies M2 kunye ne-M5 zisetyenziswa kule nkqubo [1]. Ileyibhile yeFlegi yachazwa okokuqala njenge-epitope exhomekeke kwi-calcium ye-antibody ye-monoclonal. Yi-octapeptide ene-acidic kakhulu enokuthi idibanise neprotheni ekujoliswe kuyo kwi-N-terminal. Njenge-hydrophilic peptide kakhulu, ileyibhile yeFlegi inethuba eliphezulu lomphezulu. Iprotheni yeFlegi-fusion inokubanjwa ngamakholomu e-immunoaffinity phambi kwe-Ca2 + kwaye ikhutshwe yi-EDTA kwindawo ephantsi, i-pH engathathi hlangothi kwaye ngoko ke phantsi kweemeko ze-physiological.

Iimpawu zePhysicochemical ze-FLAG peptide

Ifomula yemolekyuli C41H60N10O20

Ubunzima bemolekyuli 1012.97000

Ubunzima obuchanekileyo 1012.40000

PSA 525.79000

iflegi yePeptide TFA ityuwa. IFLAG peptide TFA SALT

Inombolo yeCAS 98849-88-8 Ubunzima bemolekyuli 1012.97000

Ubuninzi beN/A Indawo yokubila ayikho

I-molecular formula C41H60N10O20 Indawo yokunyibilikisa ayikho

I-MSDS N/A Indawo edanyazayo

FLEG Ligama lelinye igama le peptide

1.1 iflegi yePeptide
1.2 IFLEG EPITOPE PEPTIDE
1.3 I-Trypsinogen (i-pancreas yomntu isoenzyme i-2 activation peptide), i-1-L-aspartic acid-2-L-tyrosine-3-L-lysine-
1.4 iflegi ithegi yePeptide
1.5 ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
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