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  • Te hoko wera o te FLAG cas 98849-88-8

Hurae . 14, 2023 10:14 Hoki ki te rarangi

Te hoko wera o te FLAG cas 98849-88-8



111 Hokohoko FLAG cas 98849-88-8

Ko te 222 FLAG peptide he polypeptide waikawa amino e waru (asp-ty-rlys-asp-asp-asp-asp-asp-Lys) me te waahi tapahi enterokinase. Ko te FLAG peptide e whakamahia ana mo te mohio me te purenga o nga pūmua recombinant i takawaenga e nga antibodies

333 Ko te hangarau pūmua whakakotahi he taputapu nui ki te whakaoti rapanga maha e pa ana ki te hanga pūmua recombinant. Ko te ahua o te tapanga taapiri e awhina ana ki te tautuhi me te whakarato i tetahi tikanga purenga kotahi-taahiraa mo te pūmua hanumi ma te tuku i te tangohanga pūtau, supernatant ranei i roto i te pou o nga taputapu tika. Ka whakaaetia te peptide FLAG ki te ruate i raro i nga tikanga kore-denaturing. He maha nga paturopi ki te peptide FLAG kua whakawhanakehia. Ka herea te antibody M1 ki te peptide i te aroaro o te katoona whakarewa, he pai ake te Ca2 +. Whakawhanahia e te kaihoko chelating. Ko tetahi atu rautaki he whakataetae whakataetae me nga peptides FLAGe kore utu. Ka whakamahia nga antibody M2 me M5 i roto i tenei mahi [1]. Ko te tapanga Kara i whakaahuatia tuatahi hei epitope e whakawhirinaki ana ki te konupūmā o te antibody monoclonal. He octapeptide tino waikawa ka taea te hono ki te pūmua whainga i te pito-N. I te mea he peptide tino hydrophilic, he nui te tūponotanga mata o te tapanga Kara. Ka taea te hopu i te pūmua Flag-fusion e nga pou immunoaffinity i te aroaro o Ca2 + me te EDTA i te iti o te kukū, te pH kūpapa, no reira i raro i nga ahuatanga o te tinana.

Nga ahuatanga matū tinana o te peptide FLAG

tātai rāpoi C41H60N10O20

Te taumaha ngota 1012.97000

Papatipu tika 1012.40000

PSA 525.79000

FLAG Peptide TFA tote. FLAG peptide TFA TOTE

Nama CAS 98849-88-8 Te taumaha ngota 1012.97000

Kiato N/A Ira koropuke N/A

tātai rāpoi C41H60N10O20 Ira whakarewa N/A

MSDS N/A Ira kohiko

FLAG Ingoa ingoa o te peptide

1.1 FLAG Peptide
1.2 HAE EPITOPE PEPTIDE
1.3 Trypsinogen (te tangata pancreas isoenzyme 2 activation peptide), 1-L-aspartic acid-2-L-tyrosine-3-L-lysine-
1.4 Tohu Tohu Peptide
1.5 ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
Tiri
Panuku:

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Asset 3

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