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  • Hot sade FLAG cas 98849-88-8

Jul. 14, 2023 10:14 Bali menyang dhaptar

Hot sade FLAG cas 98849-88-8



111 Hot sade FLAG cas 98849-88-8

Peptida 222 FLAG minangka polipeptida asam amino wolung (asp-ty-rlys-asp-asp-asp-asp-asp-Lys) kanthi situs pemotongan enterokinase. Peptida FLAG digunakake kanggo pangenalan lan pemurnian protein rekombinan sing ditengahi dening antibodi

333 Teknologi protein fusi wis dadi alat penting kanggo ngatasi akeh masalah sing ana hubungane karo produksi protein rekombinan. Sifat label tambahan mbantu ngenali lan nyedhiyakake prosedur pemurnian siji-langkah kanggo protein fusi kanthi ngliwati ekstrak sel utawa supernatan liwat kolom substrat sing cocog. Peptida FLAG diijini kanggo eluate ing kahanan non-denaturing. Sawetara antibodi nglawan peptida FLAG wis dikembangake. Antibodi M1 ngiket peptida ing ngarsane kation logam divalen, luwih becik Ca2 +. Elusi dening agen chelating. Strategi liyane yaiku elusi kompetitif kanthi peptida FLAGe gratis sing berlebihan. Antibodi M2 lan M5 ditrapake ing prosedur iki [1]. Label Flag pisanan diterangake minangka epitope gumantung kalsium saka antibodi monoklonal. Iki minangka octapeptide asam banget sing bisa gabung karo protein target ing terminal N. Minangka peptida hidrofilik banget, label Flag nduweni kemungkinan lumahing dhuwur. Protein Flag-fusion bisa dijupuk dening kolom immunoaffinity ing ngarsane Ca2 + lan diencerke dening EDTA ing konsentrasi kurang, pH netral lan mulane ing kondisi meh fisiologis.

Sifat fisikokimia saka peptida FLAG

Rumus molekul C41H60N10O20

Bobot molekul 1012.97000

Massa akurat 1012.40000

PSA 525.79000

FLAG Peptida TFA uyah. FLAG peptida TFA SALT

Nomer CAS 98849-88-8 Bobot molekul 1012.97000

Kapadhetan N/A Titik didih N/A

Rumus molekul C41H60N10O20 Titik lebur N/A

MSDS N/A Flash point

FLAG Alias ​​saka peptida

1.1 Gendéra Peptida
1.2 BENDERA EPITOP PEPTIDE
1.3 Trypsinogen (peptida aktivasi isoenzim pankreas manungsa 2), 1-L-aspartic acid-2-L-tirosin-3-L-lysine-
1.4 tag FLAG Peptida
1.5 ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
Nuduhake
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