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Xov xwm

  • Tsev
  • FLAG CAS 98849-88-8

Lub Xya Hli. Peb 14, 2023 10:14 Rov qab mus rau npe

FLAG CAS 98849-88-8



111 Kub muag FLAG cas 98849-88-8

222 FLAG peptide yog yim-amino acid polypeptide (asp-ty-rlys-asp-asp-asp-asp-asp-Lys) nrog rau qhov chaw enterokinase-txiav. FLAG peptide yog siv rau kev lees paub thiab kev ua kom huv ntawm cov protein sib xyaw ua ke los ntawm cov tshuaj tiv thaiv.

333 Fusion protein technology tau dhau los ua cov cuab yeej tseem ceeb los daws ntau yam teeb meem ntsig txog kev tsim cov protein ntau. Qhov xwm txheej ntawm daim ntawv lo ntxiv pab txheeb xyuas thiab muab cov txheej txheem ua kom huv ib-kauj ruam rau fusion protein los ntawm kev hla cov cell extract los yog supernatant los ntawm ib kab ntawm cov substrates tsim nyog. Lub FLAG peptide tau tso cai rau eluate nyob rau hauv cov xwm txheej uas tsis yog-denaturing. Ntau cov tshuaj tiv thaiv tiv thaiv FLAG peptide tau tsim. Ib qho tshuaj tiv thaiv M1 khi rau peptide nyob rau hauv lub xub ntiag ntawm divalent hlau cation, nyiam dua Ca2 +. Elution los ntawm chelate agent. Lwm lub tswv yim yog kev sib tw elution nrog tshaj dawb FLAGE peptides. Antibodies M2 thiab M5 yog siv rau hauv cov txheej txheem no [1]. Daim ntawv cim chij tau piav qhia thawj zaug raws li calcium-dependent epitope ntawm monoclonal antibody. Nws yog ib qho acidic octapeptide uas tuaj yeem fuse nrog lub hom phiaj protein ntawm N-terminal. Raws li hydrophilic peptide heev, Flag daim ntawv lo muaj qhov ua tau zoo tshaj plaws. Flag-fusion protein tuaj yeem ntes tau los ntawm cov kab ke tiv thaiv kab mob hauv lub xub ntiag ntawm Ca2 + thiab eluted los ntawm EDTA ntawm qhov tsis tshua muaj siab, nruab nrab pH thiab yog li nyob rau hauv yuav luag physiological mob.

Physicochemical zog ntawm FLAG peptide

Cov mis mos molecular C41H60N10O20

Molecular hnyav 1012.97000

Qhov tseeb loj 1012.40000

PSA 525.79000

FLAG Peptide TFA ntsev. FLAG peptide TFA ntsev

CAS Nr .: 98849-88-8 Molecular Luj: 1012.97000

Ceev N/A Boiling point N/A

Cov mis mos molecular C41H60N10O20 Melting point N/A

MSDS N/A Flash point

FLAG Alias ​​ntawm peptide

1.1 FAG Peptide
1.2 FLAG EPITOPE PEPTIDE
1.3 Trypsinogen (tib neeg pancreas isoenzyme 2 activation peptide), 1-L-aspartic acid-2-L-tyrosine-3-L-lysine-
1.4 FLAG tag Peptide
1.5 ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
Qhia
Tom ntej:

Xov xwm tshiab
Asset 3

Xav tau kev pab?
Tso peb cov lus uas siv daim ntawv hauv qab no.

Yog tias koj txaus siab rau peb cov khoom, koj tuaj yeem xaiv tawm koj cov ntaub ntawv ntawm no, thiab peb yuav tiv tauj koj sai sai.


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