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  • I-FLAG ethengiswayo eshisayo cas 98849-88-8

Jul. 14, 2023 10:14 Buyela ohlwini

I-FLAG ethengiswayo eshisayo cas 98849-88-8



111 I-FLAG ethengisa kakhulu i-98849-88-8

I-222 FLAG peptide iyi-8-amino acid polypeptide (asp-ty-rlys-asp-asp-asp-asp-asp-Ls) enendawo yokusika i-enterokinase. I-FLAG peptide isetshenziselwa ukuqashelwa kanye nokuhlanzwa kwamaprotheni ahlangene aqondiswa amasosha omzimba.

Ubuchwepheshe be-333 Fusion protein sebuphenduke ithuluzi elibalulekile lokuxazulula izinkinga eziningi ezihlobene nokukhiqizwa kabusha kwamaprotheni. Imvelo yelebula eyengeziwe isiza ukuhlonza nokuhlinzeka ngenqubo yokuhlanza eyisinyathelo esisodwa sephrotheni ehlanganisiwe ngokudlulisela okukhishwe kweseli noma okunamandla amakhulu ngekholomu yama-substrates afanelekile. I-peptide ye-FLAG ivunyelwe ukuphuma ngaphansi kwezimo ezingezona ze-denaturing. Amasosha omzimba amaningana amelene ne-FLAG peptide akhiwe. I-antibody M1 ibophezela ku-peptide lapho kukhona i-divalent metal cation, okungcono kakhulu i-Ca2 +. I-elution nge-ejenti ye-chelating. Elinye iqhinga elokuncintisana ngama-peptide e-FLAGE amahhala. Amasosha omzimba i-M2 ne-M5 asetshenziswa kule nqubo [1]. Ilebula ye-Flag yaqale yachazwa njenge-epitope encike ku-calcium ye-monoclonal antibody. I-octapeptide ene-acidic kakhulu engahlangana nephrotheni eqondiwe ku-N-terminal. Njenge-peptide ene-hydrophilic kakhulu, ilebula ye-Flag inethuba eliphezulu lendawo. Iphrotheni ye-Flag-fusion ingathwetshulwa ngamakholomu e-immunoaffinity lapho kukhona i-Ca2 + futhi ikhishwe yi-EDTA ekugxiliseni okuphansi, i-pH engathathi hlangothi ngakho-ke ngaphansi kwezimo ezicishe zibe ngokomzimba.

Izakhiwo ze-Physicochemical ze-FLAG peptide

Ifomula ye-molecular C41H60N10O20

Isisindo samangqamuzana 1012.97000

Isisindo esinembile 1012.40000

I-PSA 525.79000

FLAG Peptide TFA usawoti. I-FLAG peptide TFA SALT

Inombolo ye-CAS 98849-88-8 Isisindo se-molecular 1012.97000

Ukuminyana N/A Iphuzu lokubilisa N/A

Ifomula yemolekyuli C41H60N10O20 Iphuzu lokuncibilika N/A

Iphoyinti le-MSDS N/A Flash

I-FLAG Isibizo se-peptide

1.1 FLAG Peptide
1.2 FLAG EPITOPE PEPTIDE
1.3 I-Trypsinogen (amapancreas womuntu isoenzyme 2 activation peptide), 1-L-aspartic acid-2-L-tyrosine-3-L-lysine-
1.4 I-FLAG ithegi ye-Peptide
1.5 ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
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