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Aug. 08, 2023 15:20 Hoki ki te rarangi

Ko Thymalin cas 63958-90-7 whatsapp:+86-19031013676



Thymalin cas 63958-90-7

He pai mo nga mate ngoikore tuatahi me te tuarua, nga mate i puta mai i te mate o te mate, te maimoatanga awhina o nga pukupuku, te anemia aplastic, te mate pukupuku mate pukupuku me te mate pukupuku, ka taea hoki te whakamahi mo te lupus erythematosus, rheumatoid arthritis, mate mate mate, etc.

Serum thymic tauwehe natura ira kohua 1658.9 + / - 65.0 ° C (Te Matapae) 1.417 + 0.06 g/cm3 kiato (Te matapae) tikanga rokiroki - 20 ° C te wairewa dChemicalbookeionizedwater: whakarea waikawa whakarewa (pKa) 3.0 8±0.10(Te Matapae) Lyophilizedpowder Tae ma

He mea nui te tauwehe thymus serum ki te whakatairanga i te rereketanga o te lymphocyte me te whakarei ake i tana mahi. Ka taea e ia te whakaihiihi i te ngohe o te pūtau aukati, te whakahaere i te mahi aukati me te whakarei ake i te mate kore-motuhake. Ka taea e nga hua matū te whakahaere i nga mahi aukati aa-a-rorohiko, me nga hua anti-pakeke, pareketo. He ahua pumau ki te wera, karekau te ngohe koiora e heke i te 80 ℃, ka ngaro te mahi koiora i muri i te hauwaitanga ki te waikawa amino. I whakamahia te tomu kuao kau hei rauemi mata, ka tangohia te ngako, te fascia me etahi atu kopa kore-thymus, ka whakamahana ki te -20 ℃, ka horoia ki te wai maru, ka kuia ki roto i te miraka miiti. E ai ki te tauwehenga papatipu o te 1: 1, ka tangohia te thymus kuru ki te wai matao mo te werohanga (ka tangohia te wai Haina me te iti o te uruhanga me te whakarewa matao), ka taea e te peptide kaha te rewa i roto i te wai me te whakapai ake i te hua. E ai ki nga purongo o nga iwi ke, ka taea hoki te tango me te saline noa) kua konatunatua, ka maka ki roto i te 10000r/min teitei-tere kiko masher, ka penupenu mo te 1min ki te hanga i te homogenize. Ko te kuao kau [whenua] → timi maru [te wai makariri mo te werohia]→[10000r/min,1min] Thymus homogenate wahi o te waiariki denaturation, centrifugation, filtration I rukuhia te thymus homogenate ka tangohia i raro i te 10 ℃, ka tio ki te -2chemical book0 ℃ mō te 48h. I muri i te rewa, whakakorikoria te pati wai me te whakamahana ki te 80 ℃, pupuri mo te 5min, kia tere te whakamatao, whakamatao ki te -20 ℃ mo te 2-3d. I muri i te rewa, i centrifuged te supernatant i 2 ℃ i 5000r/min mo 40min, a ka tātarihia i raro i te pēhanga iti (tātari penupenu ranei 0.22μm microporous membrane tātari) ki te whiwhi i te tangohanga marama o polypeptide kaha. Thymus homogenate [tangohanga i raro i te 10 ℃] → [-20 ℃, 48h] otinga mīti [80 ℃, 5min] → [5000r / min, 40min centrifuge] Hohe peptide tango ultrafiltration, purenga, tākai, lyophilization Ko te tangohanga whakamarama o te peptide kaha ko ultrafiltration membrane (e taea te pupuri i te papatipu ngota ngota iti iho i te 10,000) ki te whiwhi otinga para. Whakamaohia ki te -20 ℃. I muri i te paahitanga o te whakamatautau, ka tapirihia te 3% mannitol (excipient), ka whakahoroa, ka tātarihia, ka kohia, ka whakamaroke-tio, ka whiwhihia te thymosin mo te werohanga. Ko te tangohanga polypeptide hohe [membrane ultrafiltration]→ [Mr<10000] otinga para [te whakamaroke whakatio]→ [3% mannitol] thymosin mo te werohanga (Tuhipoka: Ko te mahi katoa me nga taputapu me whakahaere i runga i te tikanga mahi aseptic.)

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