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Aug. 08, 2023 15:20 Back to list

Thymalin cas 63958-90-7 whatsapp:+86-19031013676



Thymalin cas 63958-90-7

Iyo yakakodzera kune yekutanga uye yechipiri immune deficiency zvirwere, zvirwere zvinokonzerwa nekusashanda kwemuviri, adjuvant kurapwa kwemamota, aplastic anemia, acute uye isingaperi viral hepatitis, uye inogona zvakare kushandiswa kune lupus erythematosus, rheumatoid arthritis, allergic asthma, nezvimwe.

Serum thymic factor nature boiling point 1658.9 + / - 65.0 ° C (Zvakafanotaurwa) 1.417 + 0.06 g/cm3 density (Predicted) kuchengetedza mamiriro - 20 ° C solubility dChemicalbookeionizedwater: soluble acidity coefficient (pKa) 3.0 8±0.10(Zvakafanotaurwa) Lyophilizedpowder Ruvara ruchena

Serum thymus factor chinhu chakakosha kukurudzira lymphocyte kusiyanisa uye kuwedzera basa rayo. Inogona kukurudzira immune cell kuita, kudzora mashandiro emuviri uye kuwedzera kusiri-chaiyo cellular immune. Chigadzirwa chemakemikari chinogona kudzora cellular immune function, ine anti-kuchembera, antiviral mhedzisiro. Yakagadzikana pakupisa, chiitiko chebiological hachidzike pa80 ℃, uye chiitiko chebiological chinonyangarika mushure mekuiswa hydrolyzed kuita amino acids. Iyo mhuru thymus yakashandiswa seyakabikwa, mafuta, fascia uye mamwe asiri e-thymus tishu akabviswa, akaiswa mufiriji pa -20 ℃, akashambidzwa nemvura isina tsvina yakasvibiswa, uye akagadzika muguyo renyama. Zvinoenderana nehuwandu hwehuwandu hwe1: 1, iyo yakapwanyika thymus inotorwa nemvura inotonhora yejekiseni (mvura yeChinese inotorwa nekuderera kwemvura uye kunyunguduka kunotonhora), izvo zvinogona kuita kuti peptide inoshanda inyunguduke mumvura uye kuvandudza goho. Zvinoenderana nemishumo yekune dzimwe nyika, inogona zvakare kubviswa neyakajairwa saline) yakasanganiswa, yakaiswa mu10000r/min yakakwira-kumhanya tishu masher, uye yakashongedzwa kwe1min kuita homogenize. Mhuru thymus [pasi]→ yakapwanyiwa thymus [mvura inotonhora yejekiseni]→[10000r/min,1min] Thymus homogenate part of thermal denaturation, centrifugation, filtration The thymus homogenate yakanyoroveswa ndokubuditswa pazasi pe10℃, ndokuzoomeswa nechando pa -2chemical book0 ℃ kwe48h. Mushure mekunyunguduka, simbisai mumvura yekugezera uye kupisa kusvika ku80 ℃, chengetai 5min, tonhorera nekukurumidza, refrigerate pa -20 ℃ ye 2-3d. Mushure mekunyunguduka, iyo supernatant yaive centrifuged pa 2 ℃ pa 5000r / min ye 40min, uye yobva yasefa pasi pekumanikidzwa kwakaderedzwa (sefa pulp kana 0.22μm microporous filter membrane) kuti iwane yakajekeswa yakatorwa yepolypeptide inoshanda. Thymus homogenate [kubvisa pazasi 10 ℃]→[-20℃,48h] chando mhinduro [80℃,5min]→[5000r/min,40min centrifuge] Active peptide inobvisa ultrafiltration, kucheneswa, kurongedza, lyophilization. ultrafiltration membrane (iyo inogona kuchengetedza hukama hwema molecular mashoma kupfuura 10,000) kuwana mhinduro yakanatswa. Refrigerate pa -20 ℃. Mushure mekupasa bvunzo, 3% mannitol (excipient) yakawedzerwa, yakaomeswa uye kusefa, yakaputirwa, yakaomeswa nechando, uye thymosin yejekiseni yakawanikwa. Active polypeptide inobvisa [ultrafiltration membrane]→[Mr<10000] yakanatswa mhinduro [kuomeswa nechando]→[3% mannitol] thymosin yejekiseni (Cherechedza: Kuvhiya kwese kwese uye michina inofanirwa kuitwa zvinoenderana neaseptic operation nzira.)

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